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60105

所属分类
Kikoman
中文名称:
肌氨酸氧化酶
英文名称:
Sarcosine Oxidase
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
应用:
生化诊断
产品类别:
生化诊断:
比色法
产品描述

规格

Appearance yellow lyophilizate
Activity ≧20 U/mg
Contaminants Creatininase <1.0×10-2 U/U%
Stabilizer sucrose 
Storage condition below -20℃ protected from light

 

特性

Molecular weight ca. 49 kDa (gel filtration)
Structure monomer of 43 kDa (SDS-PAGE)
one mole of FAD per mole of enzyme
Isoelectric point 5.3
Michaelis constant 4.7×10-3M (sarcosine)
pH Optimum 6.7–9.5
pH Stability 6.5–10.5
Optimum temperature 50℃
Thermal stability below 55℃
Stability (liquid form) stable at 37℃ for at least two weeks
Stability (powder form) stable at 30℃ for at least one month
Inhibitors Zn2+,Cu2+,Hg2+,Ag+

 

应用

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

 

 

参考文献

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.

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