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60111

所属分类
Kikoman
中文名称:
肌酐酶
英文名称:
Creatininase (C1-E)
The enzyme is useful for the determination of creatinine in clinical analysis.
应用:
生化诊断
产品类别:
生化诊断:
比色法
产品描述

规格

Appearance white lyophilizate  
Activity 600-750 U/mg  
Contaminants Catalase ≦0.5%  
Stabilizer sucrose  
Storage condition below -20℃  

 

特性

Molecular weight ca. 170 kDa (gel filtration)
Structure 6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point 4.8
Michaelis constant 3.4×10-2M (creatinine)
  4.3×10-2M ((creatine)
pH Optimum 6.5–7.0
pH Stability 7.0–11.0
Optimum temperature 60–65℃
Thermal stability below 60℃
Stability (liquid form) stable at 37°C for at least two weeks 
Stability (powder form) stable at 30°C for at least one month
Inhibitors Hg2+
Activators Mg2+,Mn2+ 

 

应用

The enzyme is useful for the determination of creatinine in clinical analysis.

 

 

参考文献

Suzuki, M. and Yoshida, M. (1984)

A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system

Clinica Chimica Acta, 143, 147–155.

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