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60122

所属分类
Kikoman
中文名称:
蔗糖磷酸化酶
英文名称:
Sucrose Phosphorylase
The enzyme is useful for the determination of inorganic phosphate in clinical analysis.
应用:
生化诊断
产品类别:
生化诊断:
比色法
产品描述

规格

Appearance white lyophilizate
Activity ≧50 U/mg
Stabilizer sucrose
Storage condition below -20℃

 

特性

Molecular weight ca. 56 kDa (gel filtration)
Structure monomer of 56 kDa (SDS-PAGE)
Isoelectric point 4.6
Michaelis constant 3.9×10-2M (sucrose)
6.2×10-3M (phosphate)
pH Optimum 7.5
pH Stability 5.0–8.0
Optimum temperature 40℃
Thermal stability below 45℃
Stability (liquid form) stable at 37℃ for at least two weeks
Stability (powder form) stable at 30℃ for at least two weeks
Inhibitors glucose, glucono-1,5-lactone
Specificity sucrose (100), maltose (0), starch (0)

 

应用

The enzyme is useful for the determination of inorganic phosphate in clinical analysis.

 

 

参考文献

Koga, T. et al., Agric. Biol. Chem., 55, 1805–1810 (1991).

Kitao, S. and Nakano, E., J. Ferment. Bioeng., 73, 179–184 (1992).

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