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60131

所属分类
Kikoman
中文名称:
果糖基-缩氨酸氧化镁CET
英文名称:
Fructosyl-peptide Oxidase
The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.
应用:
生化诊断
产品类别:
生化诊断:
比色法
产品描述

规格

Appearance yellow lyophilizate
Activity ≧5.0 U/mg
Contaminants Catalase ≦1.0 U/U%
Stabilizer sodium glutamate, ethylenediaminetetraacetic acid (EDTA)
Storage condition below -20℃ protected from light

 

特性

Molecular weight ca. 60 kDa (gel filtration)
Structure monomer of 52 kDa(SDS-PAGE)
Michaelis constant 1.5×10-3M(fructosyl-valyl-histidine)
5.0×10-3M (fructosyl-glysine)
9.0×10-3M(Nε-fructosyl-lysine)
pH Optimum 7.5–8.5
pH Stability 5.5–9.5
Optimum temperature 37–45℃
Thermal stability below 45℃
Stability (powder form) stable at -20℃ for at least 12 months
Specificity fructosyl-valyl-histidine (100), fructosyl-glycine(150)
Nε-fructosyl-L-lysine (68.6)

 

应用

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

 

 

参考文献

Hirokawa K, Shimoji K, Kajiyama N. (2005)
An enzymatic method for the determination of hemoglobinA1C.
Biotechnology Letters, 27(14), 963-968.

 

Hirokawa K, Ichiyanagi A, Kajiyama N. (2008)
Enhancement of thermostability of fungal deglycating enzymes by directed evolution.
Applied Mcrobiology and Biotechnology, 78(5), 775-781.

 

Ichiyanagi A, Hirokawa K, Gomi K, Nakatsu T, Kato H, Kajiyama N. (2013)
Crystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69(2), 130-133.

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